ICS：71.100.70 Y40 团 体 标 准 T/HPCIA005-2022 代替T/HPCIA005-2021 化妆品美白功效的测定斑马鱼胚法 Cosmetics-Determinationofwhitening-Zebrafish(Daniorerio)embryo assay 2022年05月05日发布 2022年05月05日实施 广州开发区黄埔化妆品产业协会发布 全国团体标准信息平台 目录 化妆品美白功效的测定斑马鱼胚法.................................................................................................................5 1.范围.....................................................................................................................................................................5 2.术语和定义.........................................................................................................................................................5 3.规范性引用文件.................................................................................................................................................5 (一)黑色素生成灰度检测法.................................................................................................................................5 4.方法原理.............................................................................................................................................................5 5.生物模型.............................................................................................................................................................5 5.1．成鱼.................................................................................................................................................................5 5.2．鱼胚.................................................................................................................................................................6 6.材料与试剂.........................................................................................................................................................6 6.1.材料..................................................................................................................................................................6 6.2.试剂..................................................................................................................................................................6 7.仪器与设备.........................................................................................................................................................6 8.实验过程.............................................................................................................................................................7 8.1.胚胎准备..........................................................................................................................................................7 8.2.样品前处理......................................................................................................................................................7 8.3.实验方法..........................................................................................................................................................7 9.质量控制.............................................................................................................................................................7 10.结果判定...........................................................................................................................................................7 11.废弃物处置.......................................................................................................................................................8 (二)黑色素生成抑制检测法.................................................................................................................................8 12.方法原理...........................................................................................................................................................8 13.生物模型...........................................................................................................................................................8 13.1．成鱼..............................................................................