ICS13.020.01 CCSD00/09 GRM 中关村绿色矿山产业联盟团体标准 T/GRM101—2024 深色有隔内生真菌胞外代谢物生产技术规 范 ProductionProcessSpecificationsforDarkSeptateEndophytesExtracellular Metabolites 2024-12-23发布 2024-12-24实施 中关村绿色矿山产业联盟  发布 全国团体标准信息平台 T/GRM101—2024 I目次 前言.................................................................................III 1范围................................................................................1 2规范性引用文件......................................................................1 3术语和定义..........................................................................1 3.1深色有隔内生真菌darkseptateendophytes........................................1 3.2胞外代谢物extracellularmetabolite.............................................1 4产品分类............................................................................1 5要求................................................................................1 5.1人员............................................................................1 5.2生产环境........................................................................1 5.3生产设施与设备..................................................................1 5.4产品外观（感官）................................................................2 5.5产品技术指标....................................................................2 5.6成分组成要求....................................................................2 6生产技术............................................................................2 6.1菌种要求........................................................................2 6.2母种培养........................................................................2 6.3液体菌剂摇瓶培养................................................................3 6.4液体菌剂发酵培养................................................................3 6.5胞外代谢物制备..................................................................3 6.6胞外代谢物粉剂制备..............................................................3 6.7胞外代谢物胶囊制备..............................................................3 7产品参数检测........................................................................3 7.1外观测定........................................................................3 7.2浓度测定........................................................................3 7.3水分测定........................................................................3 7.4pH值测定........................................................................4 7.5粘度测定........................................................................4 7.6生长素测定......................................................................4 7.7保质期..........................................................................4 7.8胞外代谢物化合物组分测定........................................................4 8检验规则............................................................................4 8.1组批规则........................................................................4 8.2取样方法........................................................................4 8.3检验项目........................................................................4 8.4检查与验收......................................................................4 8.5判定规则........................................................................4 全国团体标准信息平台 T/GRM101—2024 II9包装、标识、运输和贮存..............................................................4 9.1包装............................................................................4 9.2标识............................................................................5 9.3运输............................................................................5 9.4贮存............................................................................5 附录A（规范性）胞外代谢物生长素含量测定比色法.......................................6 A.1方法提要........................................................................6 A.2试剂和溶液......................................................................6 A.3仪器............................................................................6 A.4标准曲线绘制.................................................