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ISO INTERNATIONAL STANDARD 16072 First edition 2002-12-15 Soil quality Laboratory methods for determination of microbial soil respiration Qualite du sol Methodes de laboratoire pour la détermination de la respiration microbienne du sol Reference number ISO 16072:2002(E) ISO @ISO2002 by IHS under lic itted without license from IHS Not for Resale ISO 16072:2002(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. @ISO2002 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's member body in the country of the requester. ISO copyright office Case postale 56 . CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland Copyriahtinteratinal organization for Standardization @IsO2002-All rightsreserved py IHS unde ermitted without license from IHS Not for Resale ISO 16072:2002(E) Contents Page Foreword Introduction 1 Scope 2 Normative references 3 Terms and definitions 4 Procedure... 4.1 General conditions. 4.2 Choice of the measuring system. 5 Measuring systems.. 5.1 Determination of O2 consumption by static incubation in a pressure-compensation system... 5.2 Determination of cO2 release by titration in a static system . 5.3 Coulometric determination of cO2 release in a static system .. 5.4 Determination of cO2 release using an infrared gas analyser in a flow-through system.. 5.5 Determination of cO2 release using gas chromatography in a flow-through system and a static system .... 10 5.6 Determination of soil respiration by pressure measurement in a static system 15 Bibliography Copyright International Organizaion for Standardization rights reserved ili nse from IHS Not for Resale ISO 16072:2002(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies (IsO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has beer International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO 16072 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological methods @ Iso 2002 - All rights reserved Copyright ionalOrganizationfor Standardization ISO 16072:2002(E) Introduction This International Standard is derived from the German standard DIN 19737 (see [1]). It describes methods for the determination of microbial soil respiration in the laboratory. Microbial soil respiration results from the mineralization of organic substances. In this process, organic substances are oxidized to the end products carbon dioxide and water, with concurrent uptake of O2 for V Not for Resale aadizao No reproductic neworkingmitedwithout licenefrmH No

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