ISO INTERNATIONAL STANDARD 16050 First edition 2003-09-01 Foodstuffs Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products High-performance liquid chromatographic method Produits alimentaires Dosage de I'aflatoxine B, et détermination de la teneur totale en aflatoxines By, B2, Gy et G2 dans les céréales, les fruits a coque et les produits derives Methode par chromatographie liquide a haute performance Reference number ISO 16050:2003(E) ISO @ ISO 2003 IHS unde Not for Resale ISO16050:2003(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In @ISO2003 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, IsO's member body in the country of the requester. ISO copyright office Case postale 56.CH-1211Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland yationagazaiSandardizatio @Iso2003-All rightsreserved Not for Resale ISO 16050:2003(E) Contents Page Foreword 1 Scope 2 Normative reference 3 Principle. 4 Reagents 5 Apparatus 6 Procedure 6.1 General 6.2 Extraction.. 6.3 Clean-up.... 6.4 HPLC operating conditions 6.5 Identification. 6.6 Calibration graph 6.7 Determination 7 Calculationofresults.. 8 8.1 Interlaboratorytest 8.2 Repeatability.. 8.3 Reproducibility 9 Test report Annex A (informative) Results of interlaboratory test. 0 Bibliography 12 ii from IHS Not for Resale ISO 16050:2003(E) Foreword IsO (the International Organization for Standardization) is a worldwide federation of national standards bodies technical committees. Each member body interested in a subject for which a technical committee has been InternationalElectrotechnicalCommission(iEC)onallmattersofelectrotechnicalstandardization International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. IsO shall not be held responsible for identifying any or all such patent rights. ISO 16050 was prepared by Technical Committee ISO/TC 34, Food products. It is based on EN 12955:1999 elaborated by CEN/TC 275, Food analysisHorizontal methods. onal Organization for Standardization @ ISO 2003 - All rights reserved ISO 16050:2003(E) INTERNATIONAL STANDARD Foodstuffs Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products High-performance liquid chromatographic method WARNING - The use of this standard involves hazardous materials and operations. This standard doesnotpurporttoaddressallthesafetyproblemsassociatedwithitsuse.Itistheresponsibilityof the user of this standard to establish appropriate safety and health practice and to determine the applicability of regulatory limitations prior to use. 1 Scope This International Standard specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatizatio