论文标题
人工眼科环境中的视网膜荧光显微镜：人源化幻影眼
Retinal fluorescence microscopy in artificial ophthalmic environment: the Humanized Phantom Eye
论文作者
论文摘要
Phantom Eye Devices的设计旨在模仿人眼的光学特性。但是，视网膜结构通常由合成组织模拟。因此，目前不可能利用幻影眼器件来进行培养物或外部组织的荧光显微镜实验，尽管这些眼科测量对于许多应用，例如测试视网膜生物标志物。在这里，我们报告并描述了旨在托管生物样品的幻影眼的发展，例如与人类诱导的多能干细胞区分开的视网膜培养物。我们表征了人源化幻象眼对标准生物标志物（例如Alexa Fluor 532和Alexa Fluor 594）的成像性能。
Phantom eye devices are designed to mimic the optical properties of the human eye; however, the retinal structures are typically emulated with synthetic tissues. Thus, it is currently impossible to exploit phantom eye devices for fluorescence microscopy experiments on cultures, or ex-vivo tissues, although these ophthalmic measurements would be of importance for many applications such as the testing of retinal biomarkers. Here we report and describe the development of a phantom eye designed to host biological samples, such as retinal cultures differentiated from human induced pluripotent stem cells. We characterized the imaging performance of the humanized phantom eye on standard biomarkers such as Alexa Fluor 532 and Alexa fluor 594.
