使用X射线激光器的纳米颗粒集合的3D衍射成像

论文标题

使用X射线激光器的纳米颗粒集合的3D衍射成像

3D diffractive imaging of nanoparticle ensembles using an X-ray laser

论文作者

Ayyer, Kartik, Xavier, P. Lourdu, Bielecki, Johan, Shen, Zhou, Daurer, Benedikt J., Samanta, Amit K., Awel, Salah, Bean, Richard, Barty, Anton, Ekeberg, Tomas, Estillore, Armando D., Giewekemeyer, Klaus, Hunter, Mark S., Kirian, Richard A., Kirkwood, Henry, Kim, Yoonhee, Koliyadu, Jayanath, Lange, Holger, Letruin, Romain, Lübke, Jannik, Morgan, Andrew J., Roth, Nils, Sato, Tokushi, Sikorski, Marcin, Schulz, Florian, Spence, John C. H., Vagovic, Patrik, Wollweber, Tamme, Worbs, Lena, Yefanov, Oleksandr, Zhuang, Yulong, Maia, Filipe R. N. C., Horke, Daniel A., Küpper, Jochen, Loh, N. Duane, Mancuso, Adrian P., Chapman, Henry N.

论文摘要

我们通过X射线单粒子成像（SPI）报告了金纳米颗粒（AUNP）的3D结构测定。使用欧洲X射线游离电子激光器的新功能，在不到100小时的光束时间内测量了来自金纳米颗粒的大约1000万个衍射图，是任何单个SPI实验中数据量的100倍以上。开发了一种分类和结构分类方法，以消除颗粒的异质性并获得比3 nm更好的分辨率。借助这些新的实验和分析发展，我们已经进入了SPI方法的新时代，并且很明显地进入生物分子的近距离原子分辨率成像。

We report the 3D structure determination of gold nanoparticles (AuNPs) by X-ray single particle imaging (SPI). Around 10 million diffraction patterns from gold nanoparticles were measured in less than 100 hours of beam time, more than 100 times the amount of data in any single prior SPI experiment, using the new capabilities of the European X-ray free electron laser which allow measurements of 1500 frames per second. A classification and structural sorting method was developed to disentangle the heterogeneity of the particles and to obtain a resolution of better than 3 nm. With these new experimental and analytical developments, we have entered a new era for the SPI method and the path towards close-to-atomic resolution imaging of biomolecules is apparent.

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