论文标题
高等植物中的线粒体具有H2不断发展的活性，这与复杂I密切相关
Mitochondria in higher plants possess H2 evolving activity which is closely related to complex I
论文作者
论文摘要
氢化酶在厌氧细菌的能量代谢中占据了中心位置。尽管线粒体复合物I的结构与氢化酶的结构相似，但它是否具有氢代谢活性尚不清楚。在这里，我们表明高等植物中存在H2不断发展的活性，与复合物I密切相关，尤其是在泛氨基结合位点附近。鱼藤酮和泛氨基酮可以抑制H2的产生。缺氧可以同时促进H2进化和琥珀酸酯的积累。根据氧气浓度通过NADH或琥珀酸酯调节的喹酮池的氧化还原性能，充当控制质子和电子流以及H2产生的阀。线粒体复合物I与代谢调节的H2不断发展活性的耦合揭示了一种更有效的氧化还原稳态调节机制。考虑到线粒体在真核生物中的普遍存在，H2代谢可能是较高生物体的先天功能。这可能至少在某种程度上解释了H2的广泛生理作用。
Hydrogenase occupy a central place in the energy metabolism of anaerobic bacteria. Although the structure of mitochondrial complex I is similar to that of hydrogenase, whether it has hydrogen metabolic activity remain unclear. Here, we show that a H2 evolving activity exists in higher plants mitochondria and is closely related to complex I, especially around ubiquinone binding site. The H2 production could be inhibited by rotenone and ubiquinone. Hypoxia could simultaneously promote H2 evolution and succinate accumulation. Redox properties of quinone pool, adjusted by NADH or succinate according to oxygen concentration, acts as a valve to control the flow of protons and electrons and the production of H2. The coupling of H2 evolving activity of mitochondrial complex I with metabolic regulation reveals a more effective redox homeostasis regulation mechanism. Considering the ubiquity of mitochondria in eukaryotes, H2 metabolism might be the innate function of higher organisms. This may serve to explain, at least in part, the broad physiological effects of H2.
